LASER CORRELATION SPECTROSCOPY (LCS) AND ITS CLINICAL PERSPECTIVES IN OPHTHALMOLOGY

Mikhail Karganov, Erika Eskina, Maria Stepanova

Abstract


The method of laser correlation spectroscopy (LCS) is based on the analysis of the spectrum of quasielastic light scatter during coherent monochromatic laser irradiation of micro-particles in biological fluids (blood serum, urine, oropharyngeal washout fluid, tear fluid etc.). Spectrum provides information on dynamic processes in the analyzed system: translation motion of scattering particles and their orientation and conformation dynamics. Special procedures of cluster analysis make it possible to find out to which linkage group a particular spectrum belongs. LCS allows evaluation of sub-fractional composition of biological fluids in a wide range of molecular sizes (from 1 to 10,000 nm), which determines principal novelty of this approach in ophthalmology.


Keywords


laser correlation spectroscopy; tear fluid; contact lenses; the cornea; PRK; Trans PRK

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References


Somov E., BrzheskijV. Tear fluid. Bibliopole Publ., 1994.

Stepanova MA, Arhipova EN, Eskina EN, Zikova AV. The prognostic significance of the study of tear fluid to assess tolerance of soft contact lenses. Oral presentation. Innovations in Science and Education, Section Modern advances in basic and clinical medicine - clinical studies. State Classic Academy. 2012; Moscow, Russia.

Vineckaja MI, Iomdina EN. The study microelements in tear liquid in certain eye diseases. Vestnik Ophthalmology. 1994; 110 (4): 24-6.

Gulari E, Tsunashima Y, Chu B. Photon correlation spectroscopy of particle distributions. J. Chem. Phys. 1979; 70(8): 3965-72.

Lebedev AD, Ivanova MA, Lomakin AV, Noskin VA. Heterodyne quasi-elastic light scattering instrument for biomedical diagnostics. Applied Optics. 1997; 36(30): 7518-22.

Hautz E, Cao A, Taillandier E et al. Conformational change of core particles studied by quasi-elastic light scattering. Biochem. 1981; 63(11-12): 891-4.

Horn DS, Dalgleish DG. Aphoton correlation spectroscopy study of size distributions of casein micelle suspensions. Eur. Biophys.1985; 11(4): 249-58.

Hwand IS, Cummins HZ. Dynamic light scattering of collagen. J. Chem. Phys. 1982; 77(2): 616-27.

Chu D. Laser light scattering. N.Y.: Acad. Press. 1974.

Gancovskij PI. About indications for intraocular correction of aphakia in diabetic patients with varying degrees of severity; 2004.

Bocharov VE, Bol'shunov AV, Gancovskij PI, et al. Laser correlation spectroscopy of tear fluid in the evaluation of the semiotic nature of postoperative inflammatory reaction and its severity in diabetic patients with implantation of intraocular lenses. Vestnik Ophthalmology. 2003; 6: 30-3.

Stepanova MA, Arhipova EN, Medvedeva US, et al. Comparative analysis of the tear fluid at different times after the operation by Trans PRK. V International scientific and practical conference "Actual problems of biology, nanotechnology and medicine". Rostov-on-Don. 2013; 120-2.

Stepanova MA, Arhipova EN, Zikova AV, et al. Evaluation of tear fluid after surgery by Trans PRK. "VII Russian scientific conference of Young Scientists " Actual problems of ophthalmology". M. 2013; 241-4.

Brzheskij VV, Somov EE. Keratoconjunctival sicca, S.-Pb., 2003.

Jefron N. Complications of wearing contact lenses and recommendations for their elimination, 1997.

Stepanova MA, Arhipova EN, Medvedeva US, et al. Evaluation of body metabolism due to prolonged use of soft contact lenses. "XIIRussian School ophthalmology".M. 2013; 395-9.

Stepanova MA, Arhipova EN, Medvedeva US, Karganov MU, Eskina EN. The role of changes in subfractional composition of tear fluid in the evaluation of the damaging effect of soft contact lenses, and excimer laser ablation of the cornea for the correction of ametropia. Pathol. Physiol. Exp. Ther. 2014; 1: 32-6.

Kurenkov VM, Majchuk DJu, Kashnikova OA. Therapy syndrome of "dry eye" before and after photorefractive surgery. Dry eye. Bibliogr. 2002; 2: 12-14.




DOI: http://dx.doi.org/10.24125/sanamed.v10i3.51

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